Attenuation of replication by a 29 nucleotide deletion in SARS-coronavirus acquired during the early stages of human-to-human transmission
Identifieur interne : 000561 ( Pmc/Checkpoint ); précédent : 000560; suivant : 000562Attenuation of replication by a 29 nucleotide deletion in SARS-coronavirus acquired during the early stages of human-to-human transmission
Auteurs : Doreen Muth [Allemagne] ; Victor Max Corman [Allemagne] ; Hanna Roth [Allemagne] ; Tabea Binger [Allemagne] ; Ronald Dijkman [Suisse] ; Lina Theresa Gottula [Allemagne] ; Florian Gloza-Rausch [Allemagne] ; Andrea Balboni ; Mara Battilani ; Danijela Rihtari [Slovénie] ; Ivan Toplak [Slovénie] ; Ram N Seage Ameneiros [Allemagne, Espagne] ; Alexander Pfeifer [Allemagne] ; Volker Thiel [Suisse] ; Jan Felix Drexler [Allemagne] ; Marcel Alexander Müller [Allemagne] ; Christian Drosten [Allemagne]Source :
- Scientific Reports [ 2045-2322 ] ; 2018.
Abstract
A 29 nucleotide deletion in open reading frame 8 (ORF8) is the most obvious genetic change in severe acute respiratory syndrome coronavirus (SARS-CoV) during its emergence in humans. In spite of intense study, it remains unclear whether the deletion actually reflects adaptation to humans. Here we engineered full, partially deleted (−29 nt), and fully deleted ORF8 into a SARS-CoV infectious cDNA clone, strain Frankfurt-1. Replication of the resulting viruses was compared in primate cell cultures as well as
Url:
DOI: 10.1038/s41598-018-33487-8
PubMed: 30310104
PubMed Central: 6181990
Affiliations:
- Allemagne, Espagne, Slovénie, Suisse
- Bade-Wurtemberg, Berlin, Canton de Berne, District de Cologne, District de Tübingen, Galice, Rhénanie-du-Nord-Westphalie
- Berlin, Berne, Bonn, Ulm
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:6181990Le document en format XML
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xml:lang="fr">Allemagne</country><wicri:regionArea>Sigmund-Freud-Str. 25, 53127 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">30310104</idno><idno type="pmc">6181990</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6181990</idno><idno type="RBID">PMC:6181990</idno><idno type="doi">10.1038/s41598-018-33487-8</idno><date when="2018">2018</date><idno type="wicri:Area/Pmc/Corpus">000479</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000479</idno><idno type="wicri:Area/Pmc/Curation">000479</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000479</idno><idno type="wicri:Area/Pmc/Checkpoint">000561</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000561</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Attenuation of replication by a 29 nucleotide deletion in SARS-coronavirus acquired during the early stages of human-to-human transmission</title><author><name sortKey="Muth, Doreen" sort="Muth, Doreen" uniqKey="Muth D" first="Doreen" last="Muth">Doreen Muth</name><affiliation wicri:level="3"><nlm:aff id="Aff1">Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117 Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.452463.2</institution-id><institution>German Center for Infection Research (DZIF),</institution></institution-wrap>Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Berlin</wicri:regionArea></affiliation><affiliation wicri:level="3"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8786 803X</institution-id><institution-id institution-id-type="GRID">grid.15090.3d</institution-id><institution>Institute of Virology,</institution><institution>University of Bonn Medical Centre,</institution></institution-wrap>Sigmund-Freud-Str. 25, 53127 Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sigmund-Freud-Str. 25, 53127 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Corman, Victor Max" sort="Corman, Victor Max" uniqKey="Corman V" first="Victor Max" last="Corman">Victor Max Corman</name><affiliation wicri:level="3"><nlm:aff id="Aff1">Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117 Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.452463.2</institution-id><institution>German Center for Infection Research (DZIF),</institution></institution-wrap>Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Berlin</wicri:regionArea></affiliation><affiliation wicri:level="3"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8786 803X</institution-id><institution-id institution-id-type="GRID">grid.15090.3d</institution-id><institution>Institute of Virology,</institution><institution>University of Bonn Medical Centre,</institution></institution-wrap>Sigmund-Freud-Str. 25, 53127 Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sigmund-Freud-Str. 25, 53127 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Roth, Hanna" sort="Roth, Hanna" uniqKey="Roth H" first="Hanna" last="Roth">Hanna Roth</name><affiliation wicri:level="3"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8786 803X</institution-id><institution-id institution-id-type="GRID">grid.15090.3d</institution-id><institution>Institute of Virology,</institution><institution>University of Bonn Medical Centre,</institution></institution-wrap>Sigmund-Freud-Str. 25, 53127 Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sigmund-Freud-Str. 25, 53127 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Binger, Tabea" sort="Binger, Tabea" uniqKey="Binger T" first="Tabea" last="Binger">Tabea Binger</name><affiliation wicri:level="3"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8786 803X</institution-id><institution-id institution-id-type="GRID">grid.15090.3d</institution-id><institution>Institute of Virology,</institution><institution>University of Bonn Medical Centre,</institution></institution-wrap>Sigmund-Freud-Str. 25, 53127 Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sigmund-Freud-Str. 25, 53127 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Dijkman, Ronald" sort="Dijkman, Ronald" uniqKey="Dijkman R" first="Ronald" last="Dijkman">Ronald Dijkman</name><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="GRID">grid.424284.c</institution-id><institution-id institution-id-type="ISNI">0000 0001 1250 5733</institution-id><institution>Federal Department of Home Affairs,</institution><institution>Institute of Virology and Immunology IVI, Bern and Mittelhäusern,</institution></institution-wrap>Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Sensemattstrasse 293, 3147 Mittelhäusern</wicri:regionArea><wicri:noRegion>3147 Mittelhäusern</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0726 5157</institution-id><institution-id institution-id-type="GRID">grid.5734.5</institution-id><institution>Department of Infectious Diseases and Pathobiology,</institution><institution>Vetsuisse Faculty, University of Bern,</institution></institution-wrap>Länggassstrasse 122, 3012 Bern, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Länggassstrasse 122, 3012 Bern</wicri:regionArea><placeName><settlement type="city">Berne</settlement><region type="région" nuts="3">Canton de Berne</region></placeName></affiliation></author><author><name sortKey="Gottula, Lina Theresa" sort="Gottula, Lina Theresa" uniqKey="Gottula L" first="Lina Theresa" last="Gottula">Lina Theresa Gottula</name><affiliation wicri:level="3"><nlm:aff id="Aff1">Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117 Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.452463.2</institution-id><institution>German Center for Infection Research (DZIF),</institution></institution-wrap>Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Berlin</wicri:regionArea></affiliation><affiliation wicri:level="3"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8786 803X</institution-id><institution-id institution-id-type="GRID">grid.15090.3d</institution-id><institution>Institute of Virology,</institution><institution>University of Bonn Medical Centre,</institution></institution-wrap>Sigmund-Freud-Str. 25, 53127 Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sigmund-Freud-Str. 25, 53127 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Gloza Rausch, Florian" sort="Gloza Rausch, Florian" uniqKey="Gloza Rausch F" first="Florian" last="Gloza-Rausch">Florian Gloza-Rausch</name><affiliation wicri:level="1"><nlm:aff id="Aff6">Noctalis, Centre for Bat Protection and Information, Oberbergstraße 27, 23795 Bad Segeberg, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Noctalis, Centre for Bat Protection and Information, Oberbergstraße 27, 23795 Bad Segeberg</wicri:regionArea><wicri:noRegion>23795 Bad Segeberg</wicri:noRegion><wicri:noRegion>23795 Bad Segeberg</wicri:noRegion><wicri:noRegion>23795 Bad Segeberg</wicri:noRegion></affiliation></author><author><name 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institution-id-type="GRID">grid.6292.f</institution-id><institution>Dipartimento di Scienze Mediche Veterinarie,</institution><institution>Facoltà di Medicina Veterinaria, Alma Mater Studiorum-Università di Bologna,</institution></institution-wrap>Via Tolara di Sopra 50, 40064 Ozzano Emilia, (BO) Italy</nlm:aff><wicri:noCountry code="subfield">(BO) Italy</wicri:noCountry></affiliation></author><author><name sortKey="Rihtari, Danijela" sort="Rihtari, Danijela" uniqKey="Rihtari D" first="Danijela" last="Rihtari">Danijela Rihtari</name><affiliation wicri:level="1"><nlm:aff id="Aff8"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0721 6013</institution-id><institution-id institution-id-type="GRID">grid.8954.0</institution-id><institution>Virology Unit, Institute of Microbiology and Parasitology,</institution><institution>Veterinary Faculty, University of Ljubljana,</institution></institution-wrap>Gerbičeva 60, 1000 Ljubljana, Slovenia</nlm:aff><country xml:lang="fr">Slovénie</country><wicri:regionArea>Gerbičeva 60, 1000 Ljubljana</wicri:regionArea><wicri:noRegion>1000 Ljubljana</wicri:noRegion></affiliation></author><author><name sortKey="Toplak, Ivan" sort="Toplak, Ivan" uniqKey="Toplak I" first="Ivan" last="Toplak">Ivan Toplak</name><affiliation wicri:level="1"><nlm:aff id="Aff8"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0721 6013</institution-id><institution-id institution-id-type="GRID">grid.8954.0</institution-id><institution>Virology Unit, Institute of Microbiology and Parasitology,</institution><institution>Veterinary Faculty, University of Ljubljana,</institution></institution-wrap>Gerbičeva 60, 1000 Ljubljana, Slovenia</nlm:aff><country xml:lang="fr">Slovénie</country><wicri:regionArea>Gerbičeva 60, 1000 Ljubljana</wicri:regionArea><wicri:noRegion>1000 Ljubljana</wicri:noRegion></affiliation></author><author><name sortKey="Ameneiros, Ram N Seage" sort="Ameneiros, Ram N Seage" uniqKey="Ameneiros R" first="Ram N Seage" last="Ameneiros">Ram N Seage Ameneiros</name><affiliation wicri:level="3"><nlm:aff id="Aff9"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1936 9748</institution-id><institution-id institution-id-type="GRID">grid.6582.9</institution-id><institution>Institute of Evolutionary Ecology and Conservation Genomics,</institution><institution>University of Ulm,</institution></institution-wrap>Albert-Einstein Allee 11, 89069 Ulm, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Albert-Einstein Allee 11, 89069 Ulm</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Tübingen</region><settlement type="city">Ulm</settlement></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="Aff10">Group Morcegos de Galicia, Drosera Society, Pdo. Magdalena, G-2, 2° esq, 15320 As Pontes, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Group Morcegos de Galicia, Drosera Society, Pdo. Magdalena, G-2, 2° esq, 15320 As Pontes</wicri:regionArea><placeName><region nuts="2" type="communauté">Galice</region></placeName></affiliation></author><author><name sortKey="Pfeifer, Alexander" sort="Pfeifer, Alexander" uniqKey="Pfeifer A" first="Alexander" last="Pfeifer">Alexander Pfeifer</name><affiliation wicri:level="3"><nlm:aff id="Aff11"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2240 3300</institution-id><institution-id institution-id-type="GRID">grid.10388.32</institution-id><institution>Institute for Pharmacology and Toxicology,</institution><institution>University of Bonn,</institution></institution-wrap>Sigmund-Freud-Str. 25, 53127 Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sigmund-Freud-Str. 25, 53127 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="GRID">grid.424284.c</institution-id><institution-id institution-id-type="ISNI">0000 0001 1250 5733</institution-id><institution>Federal Department of Home Affairs,</institution><institution>Institute of Virology and Immunology IVI, Bern and Mittelhäusern,</institution></institution-wrap>Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Sensemattstrasse 293, 3147 Mittelhäusern</wicri:regionArea><wicri:noRegion>3147 Mittelhäusern</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0726 5157</institution-id><institution-id institution-id-type="GRID">grid.5734.5</institution-id><institution>Department of Infectious Diseases and Pathobiology,</institution><institution>Vetsuisse Faculty, University of Bern,</institution></institution-wrap>Länggassstrasse 122, 3012 Bern, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Länggassstrasse 122, 3012 Bern</wicri:regionArea><placeName><settlement type="city">Berne</settlement><region type="région" nuts="3">Canton de Berne</region></placeName></affiliation></author><author><name sortKey="Drexler, Jan Felix" sort="Drexler, Jan Felix" uniqKey="Drexler J" first="Jan Felix" last="Drexler">Jan Felix Drexler</name><affiliation wicri:level="3"><nlm:aff id="Aff1">Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117 Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.452463.2</institution-id><institution>German Center for Infection Research (DZIF),</institution></institution-wrap>Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Berlin</wicri:regionArea></affiliation><affiliation wicri:level="3"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8786 803X</institution-id><institution-id institution-id-type="GRID">grid.15090.3d</institution-id><institution>Institute of Virology,</institution><institution>University of Bonn Medical Centre,</institution></institution-wrap>Sigmund-Freud-Str. 25, 53127 Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sigmund-Freud-Str. 25, 53127 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Muller, Marcel Alexander" sort="Muller, Marcel Alexander" uniqKey="Muller M" first="Marcel Alexander" last="Müller">Marcel Alexander Müller</name><affiliation wicri:level="3"><nlm:aff id="Aff1">Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117 Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.452463.2</institution-id><institution>German Center for Infection Research (DZIF),</institution></institution-wrap>Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Berlin</wicri:regionArea></affiliation><affiliation wicri:level="3"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8786 803X</institution-id><institution-id institution-id-type="GRID">grid.15090.3d</institution-id><institution>Institute of Virology,</institution><institution>University of Bonn Medical Centre,</institution></institution-wrap>Sigmund-Freud-Str. 25, 53127 Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sigmund-Freud-Str. 25, 53127 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation wicri:level="3"><nlm:aff id="Aff1">Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117 Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.452463.2</institution-id><institution>German Center for Infection Research (DZIF),</institution></institution-wrap>Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Berlin</wicri:regionArea></affiliation><affiliation wicri:level="3"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8786 803X</institution-id><institution-id institution-id-type="GRID">grid.15090.3d</institution-id><institution>Institute of Virology,</institution><institution>University of Bonn Medical Centre,</institution></institution-wrap>Sigmund-Freud-Str. 25, 53127 Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sigmund-Freud-Str. 25, 53127 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">A 29 nucleotide deletion in open reading frame 8 (ORF8) is the most obvious genetic change in severe acute respiratory syndrome coronavirus (SARS-CoV) during its emergence in humans. In spite of intense study, it remains unclear whether the deletion actually reflects adaptation to humans. Here we engineered full, partially deleted (−29 nt), and fully deleted ORF8 into a SARS-CoV infectious cDNA clone, strain Frankfurt-1. Replication of the resulting viruses was compared in primate cell cultures as well as <italic>Rhinolophus</italic> bat cells made permissive for SARS-CoV replication by lentiviral transduction of the human angiotensin-converting enzyme 2 receptor. Cells from cotton rat, goat, and sheep provided control scenarios that represent host systems in which SARS-CoV is neither endemic nor epidemic. Independent of the cell system, the truncation of ORF8 (29 nt deletion) decreased replication up to 23-fold. The effect was independent of the type I interferon response. The 29 nt deletion in SARS-CoV is a deleterious mutation acquired along the initial human-to-human transmission chain. The resulting loss of fitness may be due to a founder effect, which has rarely been documented in processes of viral emergence. These results have important implications for the retrospective assessment of the threat posed by SARS.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Jones, Ke" uniqKey="Jones K">KE Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drexler, Jf" uniqKey="Drexler J">JF Drexler</name></author><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wolfe, Nd" uniqKey="Wolfe N">ND Wolfe</name></author><author><name sortKey="Dunavan, Cp" uniqKey="Dunavan C">CP Dunavan</name></author><author><name sortKey="Diamond, J" uniqKey="Diamond J">J Diamond</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Holmes, Ec" uniqKey="Holmes E">EC Holmes</name></author><author><name sortKey="Dudas, G" 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Sci Rep</journal-id><journal-id journal-id-type="iso-abbrev">Sci Rep</journal-id><journal-title-group><journal-title>Scientific Reports</journal-title></journal-title-group><issn pub-type="epub">2045-2322</issn><publisher><publisher-name>Nature Publishing Group UK</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">30310104</article-id><article-id pub-id-type="pmc">6181990</article-id><article-id pub-id-type="publisher-id">33487</article-id><article-id pub-id-type="doi">10.1038/s41598-018-33487-8</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Attenuation of replication by a 29 nucleotide deletion in SARS-coronavirus acquired during the early stages of human-to-human transmission</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Muth</surname><given-names>Doreen</given-names></name><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-3605-0136</contrib-id><name><surname>Corman</surname><given-names>Victor Max</given-names></name><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Roth</surname><given-names>Hanna</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Binger</surname><given-names>Tabea</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0003-0320-2743</contrib-id><name><surname>Dijkman</surname><given-names>Ronald</given-names></name><xref ref-type="aff" rid="Aff4">4</xref><xref ref-type="aff" rid="Aff5">5</xref></contrib><contrib contrib-type="author"><name><surname>Gottula</surname><given-names>Lina Theresa</given-names></name><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Gloza-Rausch</surname><given-names>Florian</given-names></name><xref ref-type="aff" rid="Aff6">6</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-8049-6645</contrib-id><name><surname>Balboni</surname><given-names>Andrea</given-names></name><xref ref-type="aff" rid="Aff7">7</xref></contrib><contrib contrib-type="author"><name><surname>Battilani</surname><given-names>Mara</given-names></name><xref ref-type="aff" rid="Aff7">7</xref></contrib><contrib contrib-type="author"><name><surname>Rihtarič</surname><given-names>Danijela</given-names></name><xref ref-type="aff" rid="Aff8">8</xref></contrib><contrib contrib-type="author"><name><surname>Toplak</surname><given-names>Ivan</given-names></name><xref ref-type="aff" rid="Aff8">8</xref></contrib><contrib contrib-type="author"><name><surname>Ameneiros</surname><given-names>Ramón Seage</given-names></name><xref ref-type="aff" rid="Aff9">9</xref><xref ref-type="aff" rid="Aff10">10</xref></contrib><contrib contrib-type="author"><name><surname>Pfeifer</surname><given-names>Alexander</given-names></name><xref ref-type="aff" rid="Aff11">11</xref></contrib><contrib contrib-type="author"><name><surname>Thiel</surname><given-names>Volker</given-names></name><xref ref-type="aff" rid="Aff4">4</xref><xref ref-type="aff" rid="Aff5">5</xref></contrib><contrib contrib-type="author"><name><surname>Drexler</surname><given-names>Jan Felix</given-names></name><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0003-2242-5117</contrib-id><name><surname>Müller</surname><given-names>Marcel Alexander</given-names></name><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Drosten</surname><given-names>Christian</given-names></name><address><email>christian.drosten@charite.de</email></address><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff3">3</xref></contrib><aff id="Aff1"><label>1</label>Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117 Berlin, Germany</aff><aff id="Aff2"><label>2</label><institution-wrap><institution-id institution-id-type="GRID">grid.452463.2</institution-id><institution>German Center for Infection Research (DZIF),</institution></institution-wrap>Berlin, Germany</aff><aff id="Aff3"><label>3</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8786 803X</institution-id><institution-id institution-id-type="GRID">grid.15090.3d</institution-id><institution>Institute of Virology,</institution><institution>University of Bonn Medical Centre,</institution></institution-wrap>Sigmund-Freud-Str. 25, 53127 Bonn, Germany</aff><aff id="Aff4"><label>4</label><institution-wrap><institution-id institution-id-type="GRID">grid.424284.c</institution-id><institution-id institution-id-type="ISNI">0000 0001 1250 5733</institution-id><institution>Federal Department of Home Affairs,</institution><institution>Institute of Virology and Immunology IVI, Bern and Mittelhäusern,</institution></institution-wrap>Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland</aff><aff id="Aff5"><label>5</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0726 5157</institution-id><institution-id institution-id-type="GRID">grid.5734.5</institution-id><institution>Department of Infectious Diseases and Pathobiology,</institution><institution>Vetsuisse Faculty, University of Bern,</institution></institution-wrap>Länggassstrasse 122, 3012 Bern, Switzerland</aff><aff id="Aff6"><label>6</label>Noctalis, Centre for Bat Protection and Information, Oberbergstraße 27, 23795 Bad Segeberg, Germany</aff><aff id="Aff7"><label>7</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1757 1758</institution-id><institution-id institution-id-type="GRID">grid.6292.f</institution-id><institution>Dipartimento di Scienze Mediche Veterinarie,</institution><institution>Facoltà di Medicina Veterinaria, Alma Mater Studiorum-Università di Bologna,</institution></institution-wrap>Via Tolara di Sopra 50, 40064 Ozzano Emilia, (BO) Italy</aff><aff id="Aff8"><label>8</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0721 6013</institution-id><institution-id institution-id-type="GRID">grid.8954.0</institution-id><institution>Virology Unit, Institute of Microbiology and Parasitology,</institution><institution>Veterinary Faculty, University of Ljubljana,</institution></institution-wrap>Gerbičeva 60, 1000 Ljubljana, Slovenia</aff><aff id="Aff9"><label>9</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1936 9748</institution-id><institution-id institution-id-type="GRID">grid.6582.9</institution-id><institution>Institute of Evolutionary Ecology and Conservation Genomics,</institution><institution>University of Ulm,</institution></institution-wrap>Albert-Einstein Allee 11, 89069 Ulm, Germany</aff><aff id="Aff10"><label>10</label>Group Morcegos de Galicia, Drosera Society, Pdo. Magdalena, G-2, 2° esq, 15320 As Pontes, Spain</aff><aff id="Aff11"><label>11</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2240 3300</institution-id><institution-id institution-id-type="GRID">grid.10388.32</institution-id><institution>Institute for Pharmacology and Toxicology,</institution><institution>University of Bonn,</institution></institution-wrap>Sigmund-Freud-Str. 25, 53127 Bonn, Germany</aff></contrib-group><pub-date pub-type="epub"><day>11</day><month>10</month><year>2018</year></pub-date><pub-date pub-type="pmc-release"><day>11</day><month>10</month><year>2018</year></pub-date><pub-date pub-type="collection"><year>2018</year></pub-date><volume>8</volume><elocation-id>15177</elocation-id><history><date date-type="received"><day>16</day><month>7</month><year>2018</year></date><date date-type="accepted"><day>27</day><month>9</month><year>2018</year></date></history><permissions><copyright-statement>© The Author(s) 2018</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold> This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>.</license-p></license></permissions><abstract id="Abs1"><p id="Par1">A 29 nucleotide deletion in open reading frame 8 (ORF8) is the most obvious genetic change in severe acute respiratory syndrome coronavirus (SARS-CoV) during its emergence in humans. In spite of intense study, it remains unclear whether the deletion actually reflects adaptation to humans. Here we engineered full, partially deleted (−29 nt), and fully deleted ORF8 into a SARS-CoV infectious cDNA clone, strain Frankfurt-1. Replication of the resulting viruses was compared in primate cell cultures as well as <italic>Rhinolophus</italic> bat cells made permissive for SARS-CoV replication by lentiviral transduction of the human angiotensin-converting enzyme 2 receptor. Cells from cotton rat, goat, and sheep provided control scenarios that represent host systems in which SARS-CoV is neither endemic nor epidemic. Independent of the cell system, the truncation of ORF8 (29 nt deletion) decreased replication up to 23-fold. The effect was independent of the type I interferon response. The 29 nt deletion in SARS-CoV is a deleterious mutation acquired along the initial human-to-human transmission chain. The resulting loss of fitness may be due to a founder effect, which has rarely been documented in processes of viral emergence. These results have important implications for the retrospective assessment of the threat posed by SARS.</p></abstract><kwd-group kwd-group-type="npg-subject"><title>Subject terms</title><kwd>SARS virus</kwd><kwd>Viral evolution</kwd><kwd>Virus-host interactions</kwd></kwd-group><funding-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">https://doi.org/10.13039/501100001659</institution-id><institution>Deutsche Forschungsgemeinschaft (German Research Foundation)</institution></institution-wrap></funding-source><award-id>DFG DR 772/12-1</award-id><principal-award-recipient><name><surname>Drosten</surname><given-names>Christian</given-names></name></principal-award-recipient></award-group></funding-group><funding-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">https://doi.org/10.13039/501100007601</institution-id><institution>EC | Horizon 2020 (European Union Framework Programme for Research and Innovation)</institution></institution-wrap></funding-source><award-id>643476</award-id><principal-award-recipient><name><surname>Drosten</surname><given-names>Christian</given-names></name></principal-award-recipient></award-group></funding-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2018</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list><country><li>Allemagne</li><li>Espagne</li><li>Slovénie</li><li>Suisse</li></country><region><li>Bade-Wurtemberg</li><li>Berlin</li><li>Canton de Berne</li><li>District de Cologne</li><li>District de Tübingen</li><li>Galice</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Berlin</li><li>Berne</li><li>Bonn</li><li>Ulm</li></settlement></list><tree><noCountry><name sortKey="Balboni, Andrea" sort="Balboni, Andrea" uniqKey="Balboni A" first="Andrea" last="Balboni">Andrea Balboni</name><name sortKey="Battilani, Mara" sort="Battilani, Mara" uniqKey="Battilani M" first="Mara" last="Battilani">Mara Battilani</name></noCountry><country name="Allemagne"><region name="Berlin"><name sortKey="Muth, Doreen" sort="Muth, Doreen" uniqKey="Muth D" first="Doreen" last="Muth">Doreen Muth</name></region><name sortKey="Ameneiros, Ram N Seage" sort="Ameneiros, Ram N Seage" uniqKey="Ameneiros R" first="Ram N Seage" last="Ameneiros">Ram N Seage Ameneiros</name><name sortKey="Binger, Tabea" sort="Binger, Tabea" uniqKey="Binger T" first="Tabea" last="Binger">Tabea Binger</name><name sortKey="Corman, Victor Max" sort="Corman, Victor Max" uniqKey="Corman V" first="Victor Max" last="Corman">Victor Max Corman</name><name sortKey="Corman, Victor Max" sort="Corman, Victor Max" uniqKey="Corman V" first="Victor Max" last="Corman">Victor Max Corman</name><name sortKey="Corman, Victor Max" sort="Corman, Victor Max" uniqKey="Corman V" first="Victor Max" last="Corman">Victor Max Corman</name><name sortKey="Drexler, Jan Felix" sort="Drexler, Jan Felix" uniqKey="Drexler J" first="Jan Felix" last="Drexler">Jan Felix Drexler</name><name sortKey="Drexler, Jan Felix" sort="Drexler, Jan Felix" uniqKey="Drexler J" first="Jan Felix" last="Drexler">Jan Felix Drexler</name><name sortKey="Drexler, Jan Felix" sort="Drexler, Jan Felix" uniqKey="Drexler J" first="Jan Felix" last="Drexler">Jan Felix Drexler</name><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><name sortKey="Gloza Rausch, Florian" sort="Gloza Rausch, Florian" uniqKey="Gloza Rausch F" first="Florian" last="Gloza-Rausch">Florian Gloza-Rausch</name><name sortKey="Gottula, Lina Theresa" sort="Gottula, Lina Theresa" uniqKey="Gottula L" first="Lina Theresa" last="Gottula">Lina Theresa Gottula</name><name sortKey="Gottula, Lina Theresa" sort="Gottula, Lina Theresa" uniqKey="Gottula L" first="Lina Theresa" last="Gottula">Lina Theresa Gottula</name><name sortKey="Gottula, Lina Theresa" sort="Gottula, Lina Theresa" uniqKey="Gottula L" first="Lina Theresa" last="Gottula">Lina Theresa Gottula</name><name sortKey="Muller, Marcel Alexander" sort="Muller, Marcel Alexander" uniqKey="Muller M" first="Marcel Alexander" last="Müller">Marcel Alexander Müller</name><name sortKey="Muller, Marcel Alexander" sort="Muller, Marcel Alexander" uniqKey="Muller M" first="Marcel Alexander" last="Müller">Marcel Alexander Müller</name><name sortKey="Muller, Marcel Alexander" sort="Muller, Marcel Alexander" uniqKey="Muller M" first="Marcel Alexander" last="Müller">Marcel Alexander Müller</name><name sortKey="Muth, Doreen" sort="Muth, Doreen" uniqKey="Muth D" first="Doreen" last="Muth">Doreen Muth</name><name sortKey="Muth, Doreen" sort="Muth, Doreen" uniqKey="Muth D" first="Doreen" last="Muth">Doreen Muth</name><name sortKey="Pfeifer, Alexander" sort="Pfeifer, Alexander" uniqKey="Pfeifer A" first="Alexander" last="Pfeifer">Alexander Pfeifer</name><name sortKey="Roth, Hanna" sort="Roth, Hanna" uniqKey="Roth H" first="Hanna" last="Roth">Hanna Roth</name></country><country name="Suisse"><noRegion><name sortKey="Dijkman, Ronald" sort="Dijkman, Ronald" uniqKey="Dijkman R" first="Ronald" last="Dijkman">Ronald Dijkman</name></noRegion><name sortKey="Dijkman, Ronald" sort="Dijkman, Ronald" uniqKey="Dijkman R" first="Ronald" last="Dijkman">Ronald Dijkman</name><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name></country><country name="Slovénie"><noRegion><name sortKey="Rihtari, Danijela" sort="Rihtari, Danijela" uniqKey="Rihtari D" first="Danijela" last="Rihtari">Danijela Rihtari</name></noRegion><name sortKey="Toplak, Ivan" sort="Toplak, Ivan" uniqKey="Toplak I" first="Ivan" last="Toplak">Ivan Toplak</name></country><country name="Espagne"><region name="Galice"><name sortKey="Ameneiros, Ram N Seage" sort="Ameneiros, Ram N Seage" uniqKey="Ameneiros R" first="Ram N Seage" last="Ameneiros">Ram N Seage Ameneiros</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Attenuation of replication by a 29 nucleotide deletion in SARS-coronavirus acquired during the early stages of human-to-human transmission
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